Revel IT
A Cell Line Development Research Associate is needed for a remote contract opportunity to be involved in the creation of stable cell lines for the recombinant adeno-associated viral vector (rAAV) production processes. He/she executes experiments, produces relevant documentation, and works under supervision. He/she will be responsible for:
- Culturing mammalian cells for viral production in shake flasks, spinner flasks, spin tubes, deep well and automation platforms.
- Performing experiments in the development, optimization and scale-up of AAV producer cell lines.
- Preparing process development protocols, data analysis summaries, technical reports, and other relevant process documentation.
- Optimization of transfection and transduction protocols for newly engineered cell lines.
- Maintaining an accurate laboratory notebook.
- Ensuring work conforms to applicable quality and safety standards.
- Participating on project teams (as needed).
Education and Experience Requirements
- B.S. with 3-6 years of experience, MS.c with 2-5 years of relevant scientific or technical experience in the life sciences industry or equivalent
- Experience in cell culture including materials, instruments and technologies is essential.
- Hands-on experience in mammalian cell culture production processes is a critical requirement. This includes small scale (shaker flasks, spinner flasks, spin tubes, deep well) and/or benchtop bioreactor scale.
- Effective communication skills (verbal, written, interpersonal).
- Must be able to interact with cross-functional teams.
- Analytical and problem-solving skills.
- Ability to manage time effectively.
- Teamwork is a must.
- Must be able to lift 25 pounds.
- Due to the inherent nature of cell culture, some weekend work and/or work on holidays may be needed. On occasion, long work hours may be required depending upon the experiment schedule.
- Handling large volumes of cell culture or related materials, such as tens of liters, may be required. Also, several viral materials, including AAV may be involved in the work.
Preferred:
- Experience with viral vector production is preferred.
- Experience with creation, analysis and characterization of new engineered cell lines and stability studies (subcloning, clonal expansion, transient transfection and expression screening) is a strong plus.
- Experience in molecular biology techniques (plasmid design, molecular cloning, SDS-PAGE/western blotting, qPCR, etc) is a strong plus.
- Automation and ELN experience is a strong plus.
- Industry experience is a plus but not required
- Basic knowledge on AAV biology is a plus.
- Experience in laboratory automation hardware and software is a plus.
To apply for this job please visit www.revelit.com.
